Biologia 
 
Safety in labolatories
Gaja
Many laboratories contain significant risks, and the prevention of laboratory accidents requires great care and constant vigilance. Examples of risk factors include high voltages, high and low pressures and temperatures, corrosive and toxic chemicals, and biohazards including infective organisms and their toxins.

Measures to protect against laboratory accidents include safety training and enforcement of laboratory safety policies, safety review of experimental designs, the use of personal protective equipment, and the use of the buddy system for particularly risky operations.

What types of hazards could we deal with?
  • chemical
  • biological
  • physical (and others)
  • safety hazards - autoclaves and sterilizers, centrifuges, compressed gases, crygens and dry ice, electrical, fire

Chemical hazars
Labolatory chemicals include cancer-causing agents (carcinogens), toxins (e.g., those affecting the liver, kidney, and nervous system), irritants, corrosives, sensitizers, as well as agents that act on the blood system or damage the lungs, skin, eyes, or mucous membranes.

Biological hazard
Many laboratory workers encounter daily exposure to biological hazards. These hazards are present in various sources throughout the laboratory such as blood and body fluids, culture specimens, body tissue and cadavers, and laboratory animals, as well as other workers.

Physical hazard (and others)
Besides exposure to chemicals and biological agents, laboratory workers can also be exposed to a number of physical hazards. Some of the common physical hazards that they may encounter include the following: ergonomic, ionizing radiation, non-ionizing radiation and noise hazards.
  • Ergonimic - especialy for repetitive motion injuries during routine laboratory procedures such as pipetting, working at microscopes, operating microtomes, using cell counters and keyboarding at computer workstations.
  • Ionizing radiation - processing radioactive izotopes could pose a considerable health risk to affected workers if not properly controlled

Safety hazards
  • Autoclaves and sterilizers - workers should be trained to recognize the potential for exposure to burns or cuts that can occur from handling or sorting hot sterilized items or sharp instruments when removing them from autoclaves/sterilizers or from steam lines that service the autoclaves.
  • Centrifuges - due to the high speed at which they operate, have great potential for injuring users if not operated properly. Unbalanced centrifuge rotors can result in injury, even death. Sample container breakage can generate aerosols that may be harmful if inhaled. The majority of all centrifuge accidents are the result of user error.
  • Compressed gases - within laboratories, compressed gases are usually supplied either through fixed piped gas systems or individual cylinders of gases. Compressed gases can be toxic, flammable, oxidizing, corrosive, or inert. Leakage of any of these gases can be hazardous.
  • Crygens and dry ice - are used to produce very low temperatures [below -153 °C (-243 °F)], such as liquid nitrogen (LN2) dry ice which converts directly to carbon dioxide gas at -78 °C (-109 °F). Hand protection is required as well as eye protection.
  • Electrical - there is the potential for workers to be exposed to electrical hazards including electric shock, electrocutions, fires and explosions. Damaged electrical cords can lead to possible shocks or electrocutions.
  • Fire - is the most common serious hazard that one faces in a typical laboratory. In dealing with a laboratory fire, all containers of infectious materials should be placed into autoclaves, incubators, refrigerators, or freezers for containment.

What we could need? - Personal protective equipment

Personal protective equipment or PPE are equipments worn to prevent against exposure of hazardous substances. Although, PPE does not eliminate the risks of hazards but it helps protect the user from the exposure. To make a workplace safer, it should provide instructions and training of how to use and choose proper PPE in different situations.

PPE includes:

  • Long-sleeved shirts, lab coats, aprons.
  • goggles
  • Safety gloves; (latex or nitrile)
  • Face shield or safety

How to be safe in the lab?

  • Report all accidents, injuries, and breakage of glass or equipment to instructor immediately.
  • Keep pathways clear by placing extra items (books, bags, etc.) on the shelves or under the work tables. If under the tables, make sure that these items can not be stepped on.
  • Long hair (chin-length or longer) must be tied back to avoid catching fire.
  • Wear sensible clothing including footwear. Loose clothing should be secured so they do not get caught in a flame or chemicals.
  • Work quietly — know what you are doing by reading the assigned experiment before you start to work. Pay close attention to any cautions described in the laboratory exercises
  • Do not taste or smell chemicals.
  • Wear safety goggles to protect your eyes when heating substances, dissecting, etc.
  • Do not attempt to change the position of glass tubing in a stopper.
  • Never point a test tube being heated at another student or yourself. Never look into a test tube while you are heating it.
  • Unauthorized experiments or procedures must not be attempted.
  • Keep solids out of the sink.
  • Leave your work station clean and in good order before leaving the laboratory.
  • Do not lean, hang over or sit on the laboratory tables.
  • Do not leave your assigned laboratory station without permission of the teacher.
  • Learn the location of the fire extinguisher, eye wash station, first aid kit and safety shower.
  • Fooling around or "horse play" in the laboratory is absolutely forbidden. Students found in violation of this safety rule will be barred from particpating in future labs and could result in suspension.
  • Anyone wearing acrylic nails will not be allowed to work with matches, lighted splints, bunsen burners, etc.
  • Do not lift any solutions, glassware or other types of apparatus above eye level.
  • Follow all instructions given by your teacher.
  • Learn how to transport all materials and equipment safely.
  • No eating or drinking in the lab at any time!

Literature:
  • http://www.uvm.edu/safety/lab
  • https://en.wikipedia.org/wiki/Laboratory_safety

Vocabulary

               handling- obsługa
hazard- niebezpieczeństwo     steam lines- sterylizator parowy
contain- zawierać     centrifuge- wirówka
risk- ryzyko     speed- prędkość
prevention- zapobieganie     unbalanced- niezrównoważone
accident- wypadek     majority- większość
request- żądanie     cylinders- siłowniki/butla
constant- stały     flammable- łatwopalne
vigilance- czujność     oxidizing- utleniające
examples- przykłady     corrosive- żrące
factors- czynniki     inert- obojętne
voltages- napięcie     dry ice- suchy lód
pressure- nacisk     cryogens- ciecze kriogeniczne
corrosive- substancje żrące     liquid - płynny
toxic- tokstyczny     carbon dioxide- dwutlenek węgla
biohazard- biohazard     electrical cords- przewody elektryczne
infective- infekcyjny     shocks- wstrząsy
measure- zmierzyć     electrocutions- porażenia
protect- ochraniać     refrigerator- lodówka
against- przeciwko     freezers- zamrażarka
review- recenzja     lab coat- fartuch (labolatoryjny)
equipment- sprzęt     aprons- fartuch
autoclave- autoklaw     assure- gwarantować
sterilizers- sterylizatory     emergency- nagły wypadek
centrifuges- wirówki     involving- angażować
cryogens - ciecze kriogeniczne     waste- odpady
dry ice- suchy lód     clutter- nieład
carcinogens- rakotwórcze     contamination- zanieczyszczenie
liver- wątroba     swiftly- szybko
kidney- nerka     appropriately- odpowiednio/właściwie
irritants- drażniące     beverages- napój
blood- krew     well-ventilated- dobrze wentylowany
encounter- spotkanie/starcie     alert- alarm
exposure- ekspozycja     notify- zgłaszać
various- różnorodny         dispoze- pozbyć się
source- źródło         sink- zlew
cadavers- zwłoki         mucous membranes- błony śluzowe
repetitive- powtarzający         beaker- zlewka
injuries- szkody           flask- kolba
counters- liczniki           tongs- szczypce
autoclaves- autoklawy           ruler- linijka
sterilizers- sterylizatory           funnel- lejek
recognize- rozpoznawać           Dropper- zakraplacz
exposure- ekspozycja     Stopwatch- stoper
burns- oparzenia           minor- drobny
occur- wystąpić          chipped- wyszczerbione

Exercises
1. List 3 most unsafe activities to do in the lab and justify why.

2. True or False?

    1. The lab is a good setting for practical jokes.
    2. Spilled chemicals should be wiped up immediately.
    3. Eating and drinking in the lab is forbidden.
    4. Its is unnecessary to report minor lab accidents.
    5. Chemicals should never be tasted.
3. Match the items on the left with the correct items on the right.

 

4. Fill the gaps with words: accident alone before chemicals chipped Clean glass glassware Goggles gum hands heat Horseplay instructor location minor safety Shoes taste touch

    1. Report any ................... or injury to your instructor immediately, no matter how ................... it may appear.
    2. Never work ................... . No student may work in the laboratory without an ................... present.
    3. Do not ................... any equipment, chemicals, or other materials in the laboratory are until you are instructed to do so.
    4. No food, drink, or ................... is allowed in the laboratory. Keep ................... away from face. Do not touch, ..................., or smell any chemicals unless specifically instructed to do so.
    5. Never fool around in the laboratory. ..................., practical jokes, and pranks are dangerous and prohibited.
    6. Know the ...................and correct use of all ................... equipment.
    7. Work areas should be kept clean and tidy at all times. ................... all work surfaces and equipment ................... leaving the lab. Return all equipment clean and in working order to the proper storage area. Dispose of ................... as instructed.
    8. Long hair must be tied back and dangling jewelry and loose or baggy clothing must be secured. ................... must completely cover the foot.
    9. Never handle broken ................... with your bare hands. Use a brush and dustpan to clean up broken glass. Place broken or waste glassware in the designated glass disposal container.
    10. Examine ................... before each use. Never use ................... or cracked glassware. Never use dirty glassware. ...................must be worn when working with chemicals, ..................., or glassware.
(online: http://sciencewithsandy.com/safety/rules001.htm)

5. Match the items on the left with the correct items on the right.

 
 
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numer 3-4/2017
2017-04-01


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